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The most authoritative of artists, she seems unable, in "Rupture," to allow her audience a similar measure of authority.
During the intracellular life cycle of Listeria monocytogenes, a pore-forming toxin named listeriolysin O is largely responsible for mediating phagosomal membrane rupture to allow the pathogen escaping from the phagosome into the host cytosol [50].
However, lack of sufficient outer membrane permeability, as observed in the absence of Bax/Bak protein or with addition of poloxamer-188 in Wt mitochondria, sufficiently restrains swelling and organelle rupture to allow reestablishment of inner membrane potential and continued cell survival.
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However, the key question that remains is how the membrane is ruptured to allow the proteins to escape into the cytoplasm.
The aim of surgical treatment of acute Achilles tendon rupture is to obtain the maximum primary mechanical stability of the sutured tendon, which is crucial to ensure a low rate of postoperative re-rupture and to allow early rehabilitation.
To summarize: (1) The cell-ECM bond deformation rate regulates the development of intracellular contractility, which in turn governs adhesion rupture dynamics; (2) Rac-dependent protrusion stabilization dynamics, also regulated by ECM stiffness, contributes to cell polarization; (3) Adhesion-dependent contractile forces must rupture rear adhesions to allow faster cell migration.
Numbers in parentheses are corrections (minutes) to allow for rupture velocity.
Recordings usually started 5 min after the rupture of the membrane patch, to allow intracellular dialysis with the pipette solution.
The authors observed that the components of the cell matrix are degraded, thus exposing a loose fibrillar network, which is sufficiently weakened to allow mechanical rupture.
To allow for heterogeneous rupture velocity, we assumed the hypocenter to be the initial rupture location.
The cultures were incubated for 26 hours to allow for schizont rupture and merozoite invasion (monitored by microscopy to ensure full schizont rupture).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com