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The water contents for coexisting forsterite and enstatite in some runs were determined using unpolarized IR spectra and calculated water partition coefficients D H 2 O fo / en are between 0.01 and 0.5.
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The rate of oxygen consumption for each run was determined using Oxytherm Plus software.
Relative intensity of individual bands in the same run was determined using Phosphoimager FLA7000.
To evaluate the precision of the assay, within- and between-run variations were determined using multiple analyses of bead fractionation and MS for the two urinary samples.
Runs and tumbles were determined using the veto model: whenever any flagellum was rotating CW the cell tumbled, when all flagella were rotating CCW the cell ran.
All samples were run in triplicate and significant differences were determined using one-tailed t-test.
The oligonucleotide probe identifiers and signal intensities from the scanned microarray image files were determined using software running on the microarray scanner (Feature Extraction, v 10.5.1.1, Agilent).
P-values were determined using coalescent simulations (10,000 runs) of a standard neutral model as implemented in DnaSP.
SF and serum urea levels were determined using a specific enzymatic method run on an automated CMA600 analyzer.
Glucose concentrations were determined using a glucose standard curve run on the same plate.
Peptide masses were determined using a MALDI-TOF mass spectrometer (Micromass) running in linear mode.
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