The results obtained after each freeze and thaw cycle was expressed as a percentage change from the results for QCL-40, QCM-200 and QCH-1200 in the intra-assay run (validation run-1, these samples were prepared fresh and had not experienced any freezing conditions).
The results obtained after each freezing and thawing cycle were expressed as a percentage change from the results for QCL = 1.1 ng/mL, QCM = 15 ng/mL and QCH = 40 ng/mL in the intra-assay run (validation run-1, these samples were prepared fresh and had not experienced any freezing conditions).
To evaluate precision and accuracy, QC samples were analysed within runs (intra-run validation) at 10 replicate analyses and between runs (inter-run validation) at 10 different occasions.
An additional result of running validation plates is that they provide the researcher the characteristic standard curves (also referred to as 'calibration' or 'dilution' curves) for each target.
The assay design includes lyophylized recombinant proteins as calibrators, run-validation control samples (calibrators added to a phosphate-buffered solution), as well as a qualification panel to evaluate the analytical performance(s) in the laboratory.
The assay design included ready-to-use recombinant proteins as calibrators, run-validation control samples, and internal quality controls samples (for which target value and acceptance criteria were established in the routine setting of AD biomarker quantification).
The runs for validation used the same filtering steps described below for the production run, except where noted.
Further, the specialist patient recruitment agency runs continuous validation procedures to ensure the accuracy of their sample.
In contrast, the performance of SAC-SMA at the calibration run and the validation run is better than that of MISBA, such that R2 is 0.79 for calibration and 0.82 for validation even though it models the hydrology of UBNB in a lumped, conceptual framework as against the physically-based, fully distributed framework of MISBA.
