Sentence examples for runoff templates from inspiring English sources
The corresponding runoff templates for in vitro transcription were generated by PCR amplification.
The 5′ppp 'GC' palindromic RNA duplexes were made by in vitro runoff transcription using DNA templates (SIGMA) with a 2′ O-methyl modification on the penultimate nucleotide of the template strand [ 31].
DNA templates suitable for runoff in vitro transcription of full-length hTR, hTR(1−354), and hTR(1−208) were generated by restriction endonuclease digestion of plasmid pUC18-hTR with BamHI, StuI, and PpuMI, respectively.
A DNA template suitable for runoff in vitro transcription of hTR 353−451) was generated by restriction endonuclease digestion of plasmid pUC18-hTR 353−451) pUC18-hTR 353−451
RNA templates in Figure 2C,D were runoff transcripts from PCR products generated with different oligo combinations (Supplementary file 1) having partial complementarity to human eIF4A3 cDNA (RefSeq: NM_014740).
In runoff assays using Klenow fragment, these PNAs selectively inhibited replication of the delete template.
Stream runoff is exceedingly rare.
Thus, rivers receiving excess runoff periodically flood.
The runoff is Jan . 25and 26.
