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Wheel running was monitored using Vitalview (Mini Mitter).
Wheel running was monitored with a computer-automated system and revolutions were recorded in 1-min bins (intervals).
Spontaneous wheel running was monitored continuously during 48 h, starting at the beginning of the dark period (20 00 h), and the results obtained in the 2 days of evaluation were averaged.
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Each generation, at 6 8 weeks of age, mice are individually housed with access to a Wahman-type running wheel (circumference = 1.12 m) for 6 days, during which daily wheel running is monitored by a computer-automated system.
Sufficient convergence of chains for the Mr Bayes runs was monitored by observing log-likelihood values, the standard derivation of split frequencies (> 0.008), and diagnostics provided by AWTY [ 45, 46].
Each run was monitored for 60 h time on stream (TOS) in order to confirm the high activity and long-term stability of a bi-functional catalytic system (CuO ZnO Al2O3/H-MFI-90 CuO ZnO Al2O3/H-MFI-90 CuO ZnO Al2O3/H-MFI-90
The progress of each LC/MS run was monitored by recording the total ion current (TIC) as a function of time for ions in the m/z range of 300 to 1800.
The chromatographic run was monitored at 214 nm.
The average standard deviation of split frequencies between two simultaneous runs was monitored to confirm convergence.
The chromatographic run was monitored at 280 nm of absorbance, and after elution the fraction was lyophilized and stored at −40°C.
All analyses were repeated twice to ensure that the final trees converged to the same topology and convergence of runs was monitored with the statistics provided by MrBayes 3.2.
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