Exact(5)
Running reactions on water can reduce harmful waste and reaction times while increasing yields and reaction rates [5].
Rather than running reactions in batches one after the other, flow chemists pass starting compounds through thin, snaking tubes and carry out the needed series of reactions one after the other as the compounds travel down the molecular assembly line.
Negative controls for each qrtPCR assay were performed by running reactions without the addition of template DNA.
The SensiMix Plus SYBR (Quantace) was used to perform Real-time PCR assays running reactions on a Stratagene MX5005P thermocycler instrument.
Primer concentration was optimized for each gene in preliminary Real-Time amplification experiments by running reactions with different combinations of forward and reverse primers in the final mix (100, 300, 500 and 900 nM), then choosing those giving the highest endpoint fluorescence and a low Cq value.
Similar(55)
In this paper, several key parameters, such as reaction rate and activation energy, were tested by running reaction kinetic experiments.
The initial levels of metabolites were obtained by running reaction mixtures at time zero.
"Everything floats in the cytoplasm… We keep that internal stuff and that allows us to run reactions where a cell wall isn't necessary.
The 96-well optical plate was used to run reactions.
Phusion High Fidelity PCR Master Mix Life Technologiess) was used to run reactions which included a positive (fresh colony lysate in water) and a negative (nuclease-free water) amplification control.
No matter what type of group home you run, reaction from neighbors could be negative.
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