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KK and NO were involved in the designing and running of the microarray expriments.
JP and BF were involved in the designing and running of the microarray experiments.
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SSJ and ALBD were involved in the designing and running of the Norway/Stanford microarray experiments.
A two level, one-way ANOVA was run on the microarray expression results.
The second method was a comparison to FACS based measurements of neuronal proportion in a set of 20 bulk tissue samples run on the microarrays.
We used 300 ng of total RNA (5 μL at 60 ng/μL concentration) as starting material for each sample run on the microarray.
Tissue sample bias for running the microarray may be one of the possible reasons.
WPK, JT and CP participated in running the microarray experiments.
In order to harvest sufficient material for RNA extraction, the number of plants pooled to run the microarray experiment was different.
We wish to thank Hal Schneider, PhD, Laboratory Supervisor, and the personnel of the Children's Hospital Molecular Genetics Core facility, for service and support provided in running the DASL microarray experiments.
LB ran the microarrays, co-wrote the manuscript and co-analysed the data.
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