When simulated Ca2+ influx was run for a Ca2+ influx phase of 1 s period, the bulk cytosolic free Ca2+ rose after a delay of about 100 ms to a value of about 800 nM (Fig. 3c) in both the smooth and the wrinkled cell.
After trypsinization, cell pellets were resuspended in DAPI solution (2 mM CaCl2, 22 mM MgCl2 0.1 mg/mL BSA, 0.1% Nonidet P-40, 10 µg/mL DAPI, 10% DMSO) and run on an InFlux flow cytometer (Cytopeia).
All cell sorting experiments were performed on a BD Influx cell sorter running BD FACS Sortware.
In 2010, fuelled by the success and visibility of Sarah Palin, that looked set to change, with an influx of Republican women running for public office, according to Bonnie Grabenhofer, political director and executive vice president of National Organisation for Women.
In a time where female empowerment is running rampant and there is an influx of articles and blog posts about how incredible the female form is (particularly post-partum), I am struggling to accept my body after two babies.
There is a small influx of quarterbacks who are running threats on every down, whether from an option or a bootleg, or a pure run out of a pass formation when the pocket breaks down.
Its first line, connecting the main sites of the haj ritual, should be up and running by November, just in time for the greatest annual influx of pilgrims.
"The two main parties that have been running this country have made the country racist because of the influx that we have had," he told BBC Radio 4's The World at One. "When I've done leaflets every shopkeeper, whether they are Asian or English-born, they're concerned about the influx.
Former President Jimmy Carter says he can't see himself running for president in today's political climate, in which an unprecedented influx of new money has come to define the 2016 race for the White House.
