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Amplification-based sequencing was done in paired-end mode run to a minimum of 36 bp per read and trimmed to a minimum of 34 bp to remove low quality bases.
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But by the end of lunch I had decided it was just to keep the waiters' running to a minimum.
The approach used assumed a recessive mode of inheritance where shared variants could be determined in the affected cases and subsequently filtered out in the unaffected, while maintaining the number of sequencing runs to a minimum.
But it's great to have options – I have always lamented Spotify for not having a browser-based application, for one, since I like to keep the number of desktop apps I run to an absolute minimum.
Two lanes of Illumina HiSeq2000 sequencing were run to obtain a minimum of 30-fold coverage per allele.
"You've got to keep the runs to a minimum".
"I just want to be adequate out there, try to keep the runs to a minimum".
"Inventive fields and athletic fielding kept runs to a minimum, which meant when wickets fell Essex would always be in the game.
Spin was keeping the runs to a minimum but it was the returning Yadav who claimed the crucial wicket of De Villiers, whose charge down the pitch resulted in an uncharacteristically ungainly shot that ballooned to mid-wicket.
In the same year wind run dropped to a minimum of 100 km day−1 at Aberystwyth for ten of the twelve months whilst wind run was only less than 100 km day−1 for 2 months at Harpenden (Fig. 1h and i).
Taylor was given a life on 14 when dropped in the gully by Phil Hughes off Hussey's bowling, but other than an occasional leg-before-wicket shout there was little else in the way of chances for an Australia side desperate to keep their fourth-innings run chase to a minimum.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com