But there's a term for people who habitually, perhaps even daily, run through cycles of damaging and then purging the body: self-harm.
PCR products were purified using ExoSAP-IT (USB Corporation, Cambridge, MA), run through cycle-sequencing reactions and final products were sequenced using BigDye (Applied Biosystems, Foster City, CA) on a high-throughput capillary sequencer.
The theory is implemented in a connectionist program called ACME (Analogical Constraint Mapping Engine), which assigns an initial activation value to each possible pairing between elements in the source and target domains (based on semantic and pragmatic constraints), and then runs through cycles that update the activation values based on overall coherence (structural constraints).
Typically, at a village health post, the device would run through this cycle about three times a week, she says.
Start the dishwasher and run through a cycle.
After initial denaturation at 95 °C for 3 min, the reaction mixture was run through 35 cycles of denaturation at 95 °C for 30 s, annealing at a temperature specific for particular VNTR loci (Suppl. Table 2) for 30 s, and extension at 72 °C for 60 s, followed by a final extension at 72 °C for 5 min.
Formal publication may run through editing cycles and reviews before release.
Brand new batteries would not be affected, but as most phone batteries run through charge cycles they get less effective (this is a well-known byproduct of lithium-ion technology and one reason everyone wants to get rid of it as soon as someone figures out something better) and more susceptible to these kinds of triggering spikes.
Gene-specific primers at a final concentration of 200 nM and 1 μl of cDNA templates were combined into 20 μl reaction mixes and run through 40 cycles of PCR.
In the first part, the reaction were run through 10 cycles using the following temperature sequence: 94°C for 30 seconds to denature the DNA, 55°C for 30 seconds for annealing primes, and 72°C for 1 minute to synthesize DNA.
After initial denaturation at 95°C for five minutes, samples were run through 50 cycles of the following conditions: Denaturation for 10 seconds at 95°C and elongation at 55°C for 25 seconds.
