Sentence examples for run the following program from inspiring English sources

4. Place in thermocycler and run the following program: 30 min at 20°C, 30 min at 65°C, then hold at 4°C. ii.

Amplification was performed in Applied Biosystems GeneAmp 9700 thermocyclers running the following program: 94°C – 15' then 20 cycles of 94°C-10 94°C-105" and 72°C-10", followed by 18 cycles of 94°C-10", 57°C-50" and 72°C-10 72°C-10

Triplicate reactions were run with the following program: 94°C for 3 min; 40 cycles of 95°C for 20 sec, 57°C for 30 sec, and 72°C for 30 sec.

A PCR thermocycler was run using the following program parameters: 95°C for 30 s, 95°C for 30 s, 18 cycles (54°C for 1 min, 67°C for 20 min, 94°C for 1 min, 55°C for 1 min, 72°C for 10 min).

The slides were then placed on the DAKO (Carpinteria, CA, USA) autostainer and the following program was run: PBS/T wash followed by a 30 min incubation in 0.03% casein in PBS/T, and an 1 h incubation at room temperature with 0.5  μg ml−1 of rabbit anti-WAVE3 (New England Peptide, Gardner, MA, USA).

The following program was run: 30°C for 16 hours to run the Repli-g reaction; 95°C for 15 30 minutes to inactivate the Phi29 DNA polymerase, to activate the Taq polymerase and to melt the wax so that the Repli-g product was mixed with the PCR master mix; followed by a 45 cycle-real-time PCR with 95°C for 15 seconds and 60°C for 1 minute.

After initial denaturation for 4' at 94°C the following program was run for 35 cycles: 30″ 94°C, 3' 60°C, 1' 72°C followed by an extended elongation for 7' at 72°C.

To analyze dissociation curve profiles, the following program was run after the 40 cycles of PCR: 95 °C for 15 sec followed by a constant increase in temperature between 60 °C and 95 °C.

To analyze dissociation curve profiles, the following program was run after the 40 cycles of PCR: 95°C for 15 sec, followed by a constant increase in temperature between 60 and 95°C.

The following program was run: 50°C for 30 min, 95°C for 15 min, then 40 cycles of 94°C for 15 sec, 60°C for 1 min, followed by plate reading.

Still using the GeneAmp PCR System 2700 (ABI), the following program is run: 96°C for 2 min and 25 cycles of 96°C for 30 sec then 50°C for 15 sec then 60°C for 4 min.