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Using one eighth of each Illumina Genome Analyzer (Solexa) run sequencing capacity, the resolution with regard to CNAs lies at approximately 15 Kb with 10 fragments per bin and can be easily improved by additional sequencing.
Single reads run sequencing was done with Illumina HiSeq 2000.
The entire RAD sequencing products were resolved in two lanes of the 16-lanes per run sequencing platform Solexa HiSeq2000.
The 1st run sequencing used Illumina Miseq reagent kit (300 cycles for the 2 × 150 bp pair-end sequencing).
Sequencing sets varied in the number of lanes per run, sequencing technology, and inclusion of paired reads.
Single reads run sequencing was conducted with the HiSeq 2000.
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Each Illumina run sequenced at TGAC, both with HiSeq and MiSeq instruments, passes through this pipeline.
Dataset 2 (SRR519926) is a 2 × 250 bp run, sequenced on an MiSeq.
We then cleaned the PCR sample using Affymetrix ExoSAP-IT and ran sequencing reactions twice for each sample using Life Technologies BigDye Terminator v3.1 Cycle Sequencing Kit.
A similar result has been reported for Illumina sequencing as well, with individual runs sequenced on the same machine having dissimilar error profiles [ 11].
It can run sequences of commands at the touch of a button (turning off the music playing on the stereo and dimming the lights, for example, before starting a movie on the DVD player).
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