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BEAST runs were conducted as described above, but using 5 run replicates per cross-validation.
Convergence of run replicates was confirmed by effective sample sizes (ESS) >1200 for all parameters and by visual inspection of traces within and between replicates in Tracer v1.5.
We found good fit of posterior and prior distributions for constraints B, G, H, I, and J. Subsequently, 20 run replicates were performed with identical settings, but excluding the five unreliable constraints A, C, D, E, and F (run '-ACDEF' in Fig. 2).
The microplate reader offers the advantage of being able to run replicates of the same sample at the same time, as well as the ability to run more samples at one time (96-well plate vs. a single cuvette with the spectrophotometer) which would be useful for routine forensic casework.
The PhyloBayes CAT analysis was performed using 2 independent run replicates (40727 and 40382 generations, respectively).
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For each run, replicate concentrations of SCH 55700 were injected randomly over the immobilized surfaces to acquire association- and dissociation-phase data.
Each run, replicated 10 times, consisted of 105 iterations, after a "burn-in" of 10.
Intra-run replicates of serially diluted cDNA over the linear range of the assay demonstrated minimal variation in cycle threshold (Ct) values with a variation of <0.2 Ct, approximately corresponding to <5% variability in absolute levels.
The applicability of the proposed method is attested by its ability to improve the homogeneity of intra-run replicates amplification curves.
As shown in Table 7, digital PCR results displayed low variation both intra and inter-run replicates (% CV within-run: 4 %; overall precision: 4 %) (Table 7).> In this work, we developed for the first time a new method for the detection of miRNAs in different type of biological specimen as plasma, cell lysates or tissue.
Therefore, sex was included in the experimental design as an additional factor by running replicates separately for each sex.
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