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To confirm that cortactin was indeed knocked-down in experimental testes, Western blots were run on whole testis lysates from animals using the same reagents as those used for the dye morphological studies.
This was possibly due to a smaller sample size (fewer samples run on whole genome expression assays than miRNA assays) and/or unique susceptibility to mRNA damage in FFPE osteosarcoma tissues (compared to miRNA stability) related to the fixation or decalcification process, which might have blunted the biologic signal.
STEM profiles I, II and IV were weakly affected in UP animals but were not enriched in cytoskeletal proteins (Tables 2 3), indicating that alterations in dendritic spine numbers are not detectable in 2D-DIGE run on whole lysate samples.
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"It can run on a whole bunch of particles at once, and it's so simple".
The bean counters like the fact that you can write an application once and then, with little expense and effort, modify it to run on a whole universe of Microsoft systems from the corporate network level (Windows NT) down to a single set-top box (Windows CE).
The TBC was primarily run on the whole data set, trying to cluster the different patients.
In particular, we strongly underline that the proposed algorithm is run on the whole FVI using a common parameter setting.
Next, tRNAScan-SE 1.3.1 was run on the whole genome sequence using default parameters.
Analyses for the 2006 cohort were run on the whole year and then separately for 1 March to 31 December.
SSF 1, 4 and 8-10 werunrun on the whole pretreated slurry, while SSF 2 and 5-7 werunrun on washed pretreated slurry.
For blots run on both whole testis and seminiferous epithelium, half of each testis was prepared as described above and the other half was cut into small pieces in ice-cold PEM/250 buffer (0.2 M Pipes, 0.1M EGTA, 0.1M MgCl2, Sucrose).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com