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Finally, t-tests comparing the real circular linear correlation coefficients with the surrogate coefficients were run on sample level for each sampling point and frequency band separately.
Fig. 5 AFM images (40 μm × 10 μm) of a diamond turned surface before and after one IBP run on sample A. The amplitudes of the tool marks were reduced from 20 nm to 2.5 4 nm (height scale 80 nm).
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The qPCR reaction was run on samples known to be TINC positive and the results were consistent and analytically specific.
Cells were harvested 2 and 4 hours after cytokine re-addition and Affymetrix 430A-2.0 microarrays were run on samples harvested from each condition at 0, 2 and 4 hours.
PCR was subsequently run on samples for 40 cycles under the following conditions: denaturation (94°C for 60 s), annealing (60°C for 60 s) and extension (72°C for 90 s).
RT-PCR was then run on samples from Farms 2 5.
In total, 111 arrays were run on samples derived from 65 tissue/cell types.
Following QC, data from 104 arrays run on samples derived from 62 tissue/cell types were normalized using the robust multi-array average (RMA) expression measure [ 64].
All qRT-PCR samples were also run on samples without reverse transcriptase to confirm the product was from mRNA, not DNA, and all of these samples showed no amplicon.
qPCR arrays of MCF-7F cells were run on samples isolated from three independent experiments using triplicate Breast Cancer and Estrogen Receptor Signaling PCR Arrays as previously described (Tilghman et al. 2012).
In order to better understand the metabolite changes resulting from LPC treatment, 2D NMR experiments such as COSY, TOCSY, HSQC, and HMBC were run on samples from LPC, treated plants.
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