A simulation run generating 400,000 454 reads of length ≈250 bp (a total of 100 Mbp) takes less than 80 seconds on a 2,13 GHz single processor computer.
Each cDNA library was sequenced on a ¼ segment of a single plate run, generating 978,133 raw sequence reads for the two genotypes, totalling over 466 megabases of sequence data.
Compared to ChIPpeakAnno, GIN is able to assign each peak to a single genomic element (promoter, exon, intron or intergenic) in a single run, generating a very simple and clear output.
A total of 34.5 million reads 75 bp long (including single-end reads and paired-end reads) were obtained from one plate (8 lanes) in a single sequencing run, generating approximate 2.59 gigabase pairs (Gbp) of raw data.
Illumina sequencing can produce up to 90 Gb of data in a single run, generating reads up to 100 bp in length with an average error rate of 1-1.51-1.5%, 20].
The configuration of the filters can be also found in the nsg_backbone configuration file included in Additional file 2. The 8 pooled libraries were sequenced separately in one SOLiD run, generating a total of 260 million (M) reads of 49-bp (12.737 Gb of sequence).
