In Fig. 6, MCWT-GC-2 3, 4)GPUs/Node MCWT-GC-2 3node MCWT-GC-2 3ree, four) GPUs to run MCWT-GC-O.
Blank run of GC-MS was also performed after every sixth samples to remove traces (if any) from earlier runsin GC column.
List of Authentic standards and retention index run in GC-MS machine.
The total GC run time was 95 min.
Hold time at maximum temperature was 10 min. GC run was carried on an Agilent 7890 GC attached to an Agilent 5890 EI-single quadropole MSD.
Moreover, due to a dirt peak in the GC run we had to exclude the minor compound Z7-16 Ald from our data set.
Headspace of 1mL was injected with an autosampler (PAL, CTC, Switzerland), the GC run started at 40 °C and increased 10 °C/min up to 250 °C.
The samples were heated at 110°C in the autosampler chamber for 30 min before injection into the GC; run time was 8 min. The metabolites were quantified against a standard curve using authentic DCA, TCOH, and TCA (10 1,000 ng).
The jPAVE program's annotation module can be run to compute GC content, ORFs, SNPs, CCS against UniProt comparison, and CSS against CSS (to help detect paralogs and alternative splicing).
The initial oven temperature of 70°C was increased to 190°C at a rate of 30°C/min, subsequently to 280°C at 5°C/min and to 330°C at 20°C/min, and maintained at 330°C for 4 min (total GC run time was 28 min).
This is because the 7-min GC run per plasma sample is preceded by a short sample preparation time of 8 10 min per two samples, and ∼15 min for six samples.
