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The analyses were run at a flow rate of 0.4 mL/min, and the sample volume injected was 5 μL.
The standard size HPLC column (4.6 × 250 mm) is run at a flow rate of 1 mL/min.
The mobile phase consisted of methanol/deionized water/triethylamine (90 10 0.7, v/v/v) and was run at a flow rate of 1.0 mL/min.
Substances were separated on an endcapped, 5-µm, LiChroCART® 125-4 RP18 column (Merck, Darmstadt, Germany) run at a flow rate of 1 ml/min.
The HPLC system was run at a flow rate of 100 μL min-1 and an injected sample volume of 20 μL was used.
The samples were run at a flow rate of 0.6 ml/min at 65°C, using 5 mM H2SO4 as mobile phase.
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A HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare) was equilibrated with 15 mM sodium citrate and 150 mM NaCl, pH 5.5; the column was then loaded with the DAS-ELISA positive IEC fraction (number 3), reduced to 500 μL by Amicon Ultra-15 (MWCO 30 kDa, Millipore) and run at a flow-rate of 0.5 mL/min.
The isocratic mobile phase was comprised of water-saturated ethyl acetate run at a flow-rate of 350 μL/min.
Two columns filled with Fe(0) were used as Cr VI) removal equipment running at a flow velocity of 10 ml/min at room temperature.
The eluent system consisted of an isocratic mode of 100% (v/v) methanol, running at a flow rate of 0.5 ml/min at a column temperature of 25°C.
Samples (2 μL) were injected in methanol: water, 90:10 (v/v) mobile phase running at a flow rate of 0.35 mL/min.
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