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Each library was run at a concentration of 7 pM on one Genome Analyzer (GAII) lane using 36 bp sequencing.
Libraries were run at a concentration of 4-5 pM.
Hfq alone was also run at a concentration of 173 μM.
Amplified libraries were diluted with Elution Buffer to a final concentration of 10 nM and run at a concentration of 4 6 pM on two Genome Analyzer (GAII) lanes.
Amplified libraries were diluted with Elution Buffer to a final concentration of 10 nM and run at a concentration of 4 6 pM on 2 3 Genome Analyzer (GAII or GAIIx) lanes.
Amplified miRNA-seq libraries were diluted with Elution Buffer to a final concentration of 10 nM and run at a concentration of 4 6 pM on one Genome Analyzer (GAIIx) lanes.
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Fig. 5 a, b Shift distances of DNA fragments after gel electrophoresis run at a GO concentration of 12.5 μg/mL under different voltages.
When the reaction was run at a low concentration (0.01 M), the obtained ZnO sample exhibited the typical micro-rod structure with an aspect ratio of 18 to 30 and a diameter of 200 to 400 nm.
(ii) At least three replicates were run at a single concentration.
For the primary screen, the library was run at a single concentration, selected according to the cytotoxicity pre-screening.
The secondary assays were run at an ATP concentration of < KM for ATP, such that the IC50 value measured approximates to Ki, thereby providing an accurate assessment of selectivity.
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