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Cells remaining on the glass surface (adhesive cells) were removed using a rubber scraper and suspended in 3 ml of water.
Cultured cells were harvested with a rubber scraper and washed twice with cold phosphate-buffered saline (PBS).
Six h later the cultures were washed twice with ice-cold PBS, and the cells were detached using a rubber scraper.
The cells were washed with PBS and collected with a rubber scraper.
Adherent cells were scraped off with a rubber scraper and further incubated with the extracting solution on ice for 15 min with intermittent vortexings.
For STHdh cell lines, cells were grown in 10 cm dishes to confluency, washed with PBS and collected using a rubber scraper.
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Disadvantages resulting from using the small slope are overcome by using the rubber scrapers.
Using rubber scrapers in DSSSHS model enhanced the total internal heat transfer coefficient (h1) as well as the productivity.
Results of the two models were compared to evaluate the advantages of using rubber scrapers in the new model.
In this study, a new double-slope solar still hybrid with rubber scrapers (DSSSHS) and a double-slope solar still (DSSS) were designed, manufactured and tested.
Then, 6 hr post-plating, cells were scratch wounded with a trimmed rubber cell scraper to generate an ∼500-μm-width wound.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com