Sentence examples for rubber policeman from inspiring English sources

For the western blot assay cell lysates of hMVEC were prepared by scraping the wells on ice using a rubber policeman and by adding Roche lysis buffer with phosphatase and protease inhibitors (Roche, Mannheim, Germany).

KB cells were scraped with a rubber policeman, and pelleted.

The cells were rinsed twice with PBS and were scraped with a rubber policeman in PBS.

After overnight incubation, a scraped area was made by a rubber policeman.

Cells were removed from the upper part of the membrane with a rubber policeman.

Cells were washed in cold PBS, scraped with a rubber policeman and spun down for 5 min at 2500 rpm.

Cells were washed twice with ice-cold phosphate-buffered saline (PBS) and thereafter collected using a rubber policeman.

These cells were infected with C. pneumoniae and lysed by mechanical disruption with a rubber policeman 72 hour post infection.

For preparation of nuclear protein, human podocytes were washed twice with cold phosphate-buffered saline (PBS) and scraped off the plate with a rubber policeman.

For Western blot analysis, cells were grown in 6-well plates, washed with PBS, harvested with a rubber policeman, and collected by brief centrifugation.

Whole cellular extracts from control and stimulated cells were prepared from cells detached using a rubber policeman, and harvested from culture medium into Eppendorf tubes.