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In addition, a negative control against genomic contamination was carried out by performing RT without any primer in RT.
In order to investigate, if the hot start RT lead to an increase in analytical sensitivity of the MERS-CoV assay, a half-logarithmic serial dilution of the MERS-CoV IVT ranging from 100 to 0.1 copies per reaction was analyzed with a reaction mix containing hot start RT (25 μM aptamer) and standard RT (without aptamer).
Hypofractionated RT without ADT was associated with increased risk of OM, CSM, and PSA failure as compared with other treatments except RT alone (low or high dose), as with toxicity outcomes.
XRD measurements affirm that nanocrystalline TiO2 film with the anatase phase could be grown at RT without any apparent contamination.
They were designed to act directly against HIV-1 RT without the need for prior activation (phosphorylation).
Each experiment was performed at least twice following the identical conditions using controls of only Cd II) solution and adsorbents (MITDC and RT) without metals in solution.
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Control amplification was done either without RT or without RNA.
Control amplifications without RT or without a cDNA template did not produce any PCR products.
Recently, however, Zehetleitner and colleagues [38], [39], [62] have shown that a reduction in feature contrast can lead to slower RTs without making the search inefficient.
cDNAs were run in duplicates, including non-RT (without reverse transcriptase) and no-template controls.
Control reactions "RT-" (without the enzyme RT) and "H2O", without RNA, were carried out.
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