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and 10% of the ruthenium catalyst [Ru], in 100 mM phosphate buffer pH 7.5 and 10% DMSO in the presence of ct-DNA thus led to the desired parent compound 4 after only 5 min at rt (clean conversion by HPLC).
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After hydrolysis, RT reactions were cleaned using the MinElute Cleanup kit (Qiagen).
Clones for each RT domain were than cleaned up using ExoSAP-IT (USB Corporation), and used for Sanger sequencing to verify presence of specific RT domains in the clones.
(c)These cells (five of the six leukemic cell lines) express an RNA transcript, which when amplified by RT-PCR gave a clean band of 692bp which is consistent with the predicted size for EPCR specific transcripts.
The films are deposited on single-crystal (111) n-type silicon wafers, glass and carbon-coated Cu grids (for TEM) at two different temperatures: (1) RT and (2 200°CC inside a clean stainless steel vacuum chamber with a background pressure of 5 × 10−6 mbar.
For example, 'multi-rt' denotes the AM retrained using dereverberated multi-condition data, and 'cln+mlt-rt' denotes the AM retrained using clean and processed multi-condition data.
Following RT, single-stranded RNA was treated with RNAse A. Then, RT reactions were cleaned using Illustra CyScribe GFX purification Kit (GE Healthcare).
Subsequently, RT-PCR products were cleaned using the MinElute PCR purification kit (Qiagen).
For the Behavioral study, RTs were calculated from cleaned files using an in-house Matlab program (Matlab 7.80; R2009a; The MathWorks, Natick, MA, USA) (Nelles et al. 2003).
crossTen-x3/ 4226 2 years jhr working on merge: ertest clean up, move ertest tests into rtest.
curl2-x7/ 4226 2 years jhr working on merge: ertest clean up, move ertest tests into rtest.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com