Sentence examples for rrl in from inspiring English sources
The mRNAs were added to rabbit reticulocyte lysate (RRL) in the presence of translation elongation inhibitors.
To determine if GLV IRES requires a pre-initiation complex for translation initiation, we tested 5' capped dicistronic constructs in RRL in the presence of edeine and we found that the GLV IRES mediated translation was unaffected up to 0.5 µM of the drug (Fig. 4C), whereas the cap-mediated as well as the EMCV IRES mediated translation was inhibited by edeine in a dose dependent manner (Fig. 4A).
Translation reactions contained 0.63 μg of RNA, 10 units of RNase inhibitor, and 8.8 μL of RRL in 12.5 μL.
In fact, for Figure 4, presenting pretranslocation toeprints with RRL in which eEF2 is specifically inhibited by diphtheria toxin would be more appropriate than those with yeast ribosomes.
In avipox, the TK gene and the adjacent HT-motif protein gene (HT) are in the location occupied by ribonucleotide reductase, large subunit (RRL) in orthopox and suipox.
Performing high-throughput sequencing to a sufficient depth on sheared RRL in a pooled DNA sample in the first place enables reconstruction of the sampled genome fraction by de novo sequence assembly.
Both geneticin and gentamicin decreased ribosomal accuracy in cell-free translation assays (RRL) and in HEK cells in a dose-dependent manner.
(B ) Direct telomerase activity assay to test the rescue of WT and K78E telomerases produced in RRLs, in the absence of POT1-TPP1 (No PT) or WT, E169K, and E215K TPP1 in complex with wild-type POT1.
It seemed that the Fluc domain in FG was correctly folded when synthesized in RRL, but not in S30.
As PTB is a well known regulator of picornavirus translation [13] its effect on FCV translation was assessed by titration of recombinant his-PTB in a rabbit reticulocyte lysate (RRL) FCV in vitro translation assay (Fig 8).
The rRNA operon alone contains 11 introns (eight in rrl and three in rrs), whereas the remaining genes contain one (psaB and trnL uaa)), two (psbC), or four (psbA) introns (for their predicted positions, sizes, and assigned subgroups, see table 4).
