The most efficient configuration was a rotating disk speed of 21 rpm using a step-type metering gap, resulting in C¯R= 14.7%, Ew = 84.7% and Q = 1076.6 kg/h.
SU-8 (1.25 mL NANO SU-8 100, MicroChem, US) was spin-coated onto the glass wafer in two steps: 500 rpm for 10 s with a speed ramp of 100 rpm s−1, and 1000 rpm for 30 s with a speed ramp of 300 rpm s−1.
The pupae homogenates were centrifuged in following steps at 3,000 rpm for 30 min, 6,000 rpm for 30 min, 12,000 rpm for 60 min, and 18,000 rpm for 60 min at 4°C.
After removing unreacted diethylene glycol bis (3-amino propyl) ether by two centrifugation steps (6000 rpm for 30 min), 1.5 mL of Au NP clusters (7.1 × 109 particles/mL) re-dispersed in Milli-Q water (18.2 MΩ) were added to 0.05 g/mL SNARF succinimidyl ester in Milli-Q water (18.2 MΩ) on ice, followed by stirring at room temperature overnight without exposure to light.
Hybridization was carried out for 17 hours at 65°C at 10 rpm, followed by washing steps.
Bacteria were subsequently harvested by differential centrifugation (1st step: 800 x g for 5 min to remove mammalian cells, 2nd step: 4400 rpm for 10 min).
The solution was deposited on the mp-TiO2 layer by spin-coating in two steps at 1000 rpm for 10 s and 4000 rpm for 30 s.
A perovskite layer was formed on UC-TiO2 layer by spin-coating perovskite precursor solution in two steps at 1000 rpm for 10 s and 4000 rpm for 30 s, and 200 μL chlorobenzene was poured on the substrate during the second step before the end of 20 s.
Nuclei and cellular debris were removed by centrifugation steps of 3,000 rpm, 5 min, 4°C and then 14,000 rpm, 5 min, 4°C).
After a second centrifugation step (3600 rpm for 15 minutes), the bead solution was re-suspended in PBS (pH 7.4) with the addition of 100 µg of the corresponding protein (InlA, fibronectin, FITC-fibronectin).
After another centrifugation step (1,000 rpm, 4 °C, 5 min) cells were incubated at 4 °C in hypotonic propidium iodide buffer as described before [ 32].
