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The 450 nucleotides that encode the carboxyl-terminus of the nucleoprotein (N-450) are routinely sequenced for genotype analysis.
Direct sequencing of 148 of these DArT markers identified 30 putative loci including four routinely sequenced for evolutionary studies in plants.
TBC was run either on the whole genome (≈9,000 bases) or restricting on the polymerase gene (≈2700 bases), which is the routinely sequenced gene in clinical practice for drug-resistance testing.
Modified slightly for systematics above the species-level the categories are: This encompasses: a) universality- which loci can be routinely sequenced across Lepidoptera; and b) sequence quality- which loci are most amenable to the production of bidirectional sequences with few or no ambiguous base calls?
The PCR products were routinely sequenced to confirm amplification of the targeted sequence.
We routinely sequenced a total of 192 or more per library.
Similar(47)
The Laboratory routinely sequences from 16s ribosomal bacterial DNA for identification.
The suggestions caused outrage at the time; but routinely sequencing a baby's genome at birth will be possible in a decade.
One of the first studies to explore the idea of routinely sequencing the genes of newborns to help guide their health care has run into an unexpected road bump: Few parents approached are interested in having their baby's genome profiled.
With the high quality of the final sequence data that are produced with our technique, we conclude that the presented method is suitable for routinely sequencing complete influenza A virus genomes with a high throughput.
Capturing information on all 8 avian influenza virus gene segments by routinely sequencing wild bird isolates would enhance understanding of avian influenza virus dynamics even more.
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