Sentence examples for routinely passage from inspiring English sources

Because of these concerns, some investigators routinely passage recombinant strains through mice or other animals before conducting a definitive quantitative virulence assay in an animal model [13].

Furthermore, cells were routinely passaged every 2 days at a density of 0.5~1.0 × 106 cells/mL in shake flasks in a humidified incubator maintained at 37 °C and 5%% CO2 with shaking at 140 rpm.

To further advance the knowledge in this field, hMSCs were cultured and routinely passaged on tissue-culture polystyrene to investigate the correlation between cell stiffening and differentiation potency during in vitro aging.

Cells were routinely passaged at 2 to 3 day intervals.

These established monolayers of RPE-like cells were routinely passaged every 2 4 weeks and have undergone six passages to date.

AFCs were routinely passaged using 0.05% Trypsin/EDTA (Invitrogen) at varying ratios of 1∶3 or even 1∶6 when cells were sub-confluent.

Cells were kept in logarithmic growth phase by routinely passaging them twice a week and were plated 24 h prior to treatments.

Cell lines were routinely passaged by aspirating the colonies into fine, glass pipettes and transferring the resultant disaggregated cells to fresh wells or by dissociation with Cell Dissociation Buffer.

Unlike mouse embryonic stem cells which can be efficiently expanded and differentiated from single cells, hESCs are routinely passaged as small clumps of cells or differentiated via embryoid bodies formed from tens to hundreds of cells [1].

No differences in the expression of the pluripotent marker Oct4 (Fig. 1C D) were found between cells cultured in 96-well plates and hESCs routinely passaged in medium conditioned on MEF in larger dishes (35-mm or 60-mm dishes).

Once established, rES cells were routinely passaged every 3 4 days up to 5 passages in RESM in the presence of rat LIF (Millipore/Chemicon International) at 1000 U/ml, and then they were cultured in RESM supplemented with 250 U/ml, 500 U/ml or 1000 U/ml rat LIF (Ws-4 rES cell line) or without rat LIF (Ws-9 rES cell line).