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"It's a green, soupy mess," said Martha McCann, president of a local homeowners' group, who faults the county not only for having introduced the plants but also for not routinely maintaining the site.
For stock animals, we cultured the animals according to standard methods (Brenner 1974), routinely maintaining the strains at 18° on nematode growth medium) plates with a lawn of Escherichia coli (OP50).
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In the analysis chamber, pressures of 1~2 × 10−7 Pa were routinely maintained.
In the analysis, chamber pressures of 1 ~ 2 × 10-7 Pa were routinely maintained.
NIH3T3 was routinely maintained in a DMEM medium, while all the other cell lines were cultured in a 1640 medium.
Shiraia sp. SUPER-H168 was routinely maintained on PDA slants at 4 °C by regular sub-cultivation (no longer than 3 months).
The pure culture was routinely maintained on LB (Luria Bertani, Merck, Whitehouse Station, NJ, USA) agar at 28 °C or cultivated in LB broth agitated at 220 rpm.
Pure cultures were routinely maintained at 4°C on YPD slants, and the stocks were stored at −80°C with glycerol at a final concentration of 40% (v/v).
The strain was sub-cultured at an interval of 4 5 weeks and routinely maintained on Potato Dextrose Agar (PDA) slants under refrigerated conditions.
It was routinely maintained at 4 °C on potato dextrose agar (PDA) (g/L: potato infusion from 200 g potatoes, dextrose 20, yeast extracts 0.1, agar 20, pH 5.0).
The fidelity of replication is experimentally validated to be extremely good and deviations are routinely maintained with 10% of the fabricated NHA depths.
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