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Using these conditions, the efficiency of transfection was routinely in the range of 30 to 40% as determined by immunofluorescence (GFP positive).
With these conditions, the yield of CF produced mAQP4 M23 in 50 µl analytical scale micro-reactor reactions as well as in 3 ml preparative scale maxi-reactor reactions were routinely in the range of 1.5 2 mg protein per one ml RM.
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The spatial resolution that can be routinely obtained is in the range of sub-μm (SIMS) to ~15 μm (MALDI).
State-of-the-art mass spectrometers can detect analytes routinely in the femtomolar-attomolar range.
Based on > 80 published human biomonitoring studies, it has been estimated that unconjugated BPA was routinely detected in blood in the range of nanograms per milliliter, which is similar to the range of conjugated BPA detected in the urine samples (Vandenberg et al. 2010).
Concentrations of glyphosate entering surface waters are not routinely monitored, but values in the range of 10 15 μg/L have been reported in rivers (e.g. [ 8, 9]), while measurements in the high μg/L range have been recorded only occasionally and are generally associated with direct application to wetland environments [ 7, 10].
The main advantage of MS is sensitivity - it can detect analytes routinely in the femtomolar to attomolar range.
The main advantage of MS is sensitivity, as state-of-the-art mass spectrometers can detect analytes routinely in the femtomolar to attomolar range.
Ground-based instruments such as radars and lidars have also been used routinely for diurnal tide observations in the range of 80 and 120 km (Fritts and Isler 1994; Vincent et al. 1988, 1998; Yi 2001; Manson et al. 2002; She et al. 2004; Zhang et al. 2004; Yuan et al. 2006, 2008; Lu et al. 2012; Huang et al. 2012, 2013a, [b]; Gong et al. 2013).
2.5 μM was chosen because this concentration is in the range used routinely for studying its effect on p38 activity in cellular systems (Haq et al. 2002; Wang et al. 2002; Iwasa et al. 2003; Davis et al. 2005).
To our knowledge, no other studies have evaluated the association between As exposure and DNA repair gene expression or protein levels in human populations, particularly at As levels that are in the range that is routinely found in the United States.
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