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Additional lung samples were fixed in 4% buffered paraformaldehyde (PFA; pH 7.4) and were routinely embedded into paraffin wax.
Tissue samples were fixed in 10% buffered formalin, processed routinely, embedded in liquid paraffin and sectioned at 3 µm.
Following PME, all phosphate-buffered formalin-fixed samples were routinely embedded in paraffin wax. 4 µm thick sections, cut on a rotary microtome, were stained with haematoxylin and eosin or used for IHC detection of influenza A nucleoprotein by the avidin-biotin-peroxidase complex method [33] (see Protocol S1).
The PFA-fixed material was routinely embedded in paraffin.
Tissues were routinely embedded in paraffin after 10% formalin fixation.
The tissue samples were routinely embedded in paraffin and 4 μm sections were cut.
Similar(13)
Indeed, those implementing self-screening should routinely embed a robust evaluation to underpin the ongoing use of resources in this area.
Finding effective ways to encourage health professionals to routinely embed high-quality clinical evidence into their everyday work is important, but has proved a major challenge.
"For example, industrial pumps will routinely contain embedded computational intelligence.
Formalin-fixed tissues were routinely processed, embedded in paraffin, and sectioned before staining with haematoxylin & eosin, Periodic acid-Schiff, and/or picrosirius red stains.
Tissues were routinely processed, embedded in paraffin and sectioned.
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