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Thus, using routinely authenticated strains with a known genetic pedigree is worthwhile.
The stocks were routinely authenticated on the basis of viability, growth rate and morphology by microscopic examination.
They were routinely authenticated on the basis of their viability, growth rate, and morphology by microscopic examination.
The MCF-7 and MDA-MB-231 human breast cancer cell lines were purchased from the American Type Culture Collection (ATCC) and were routinely authenticated with genotype profiling according to ATCC guidelines.
Cells were routinely authenticated every 6 months (last examined in February 2012) by growth curve analysis, cell morphology monitoring and testing for mycoplasma (R&D Systems' new MycoProbe Mycoplasma Detection Kit).
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ATCC routinely authenticates these lines by isoenzyme (interspecies) and STR (intraspecies) analysis.
The cell culture collections mentioned above routinely authenticate their cell line stocks and provide a Certificate of Analysis, including an STR profile, for each line they produce.
All in-house cells were authenticated and are routinely verified as endocrine resistant.
Cells were routinely tested for mycoplasma contamination and authenticated by short tandem repeat profiling.
The cells were routinely checked for mycoplasma infections and authenticated using the Powerplex 16 kit (Promega, Madison, WI).
Cells were authenticated by PCR from microsatellites and routinely confirmed by morphology, PCR and immunoblotting.
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