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At least three routine sputum samples were collected from each patient into 50 ml screw-cap centrifuge tubes (Falcon, Becton Dickenson, USA) prior to treatment initiation as well as during treatment as follow-up control samples.
Studies on prevalence within adult CF patients are limited; with one group finding ten percent of CF patients had cultivatable isolates from routine sputum samples during periods of clinical stability when specifically sought using enhanced culture techniques [ 2].
This was performed using routine sputum samples from routine TB patients attending Mulago National Referral Hospital, which were tested in parallel with the NTRL from March to June 2009.
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Expectorated sputum samples (following routine spirometry) were collected for adult patients.
The LRPS assay is a sensitive, high-throughput technique with potential application to routine genotyping of MTBC in sputum samples from TB patients.
No other recognized LRTI bacterial pathogen was identified in 51 of the 64 samples for which routine sputum culture results were available.
A further 2 sputum samples were collected for routine fluorescence smear microscopy after centrifugation.
Sputum samples submitted for the routine Ziehl Neelsen staining for diagnostic purpose were used for bacteriological examination.
All spontaneously expectorated sputum samples collected from patients, during both routine and non-routine visits as well as hospitalisations, were analyzed by the McGill University Health Centre clinical microbiology laboratory according to standard protocols for CF samples.
S. aureus strains isolated from sputum samples were collected from January 2006 to December 2006 using routine laboratory culture methods and standard identification methods [ 50, 51].
Sputum samples were processed by the NHLS for routine TB diagnosis by smear microscopy and culture.
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