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The remaining 2 flocks were broiler flocks where 2 Salmonella serovars were found in the routine sock samples from each flock.
From 2 flocks, where Salmonella was found in 4 and 5 of the routine sock samples, respectively, Salmonella was only detected in one 5-sock-sample.
Whenever Salmonella was detected in routine sock samples, either the person in charge of the broiler flock or the local District Veterinary Officer was contacted in order to obtain follow-up samples for this study.
Legends: See Table 3 For the 26 flocks the time elapse between receiving the routine sock samples and the 5-sock samples was in the range of 10 24 days, with "time elapse peaks" at 14, 15, 16 and 20 days, for 5, 3, 4 and 5 flocks, respectively (data not shown).
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In 16 flocks, where Salmonella was detected in the routine samples (ranging from 1 to 4 Salmonella positive sock samples), Salmonella was not detected in 5-sock-samples.
The surface areas where 12 wipe samples were collected, corresponding to 12 of 13 HEPA vacuum sock samples, were immediately sampled again with HEPA vacuum sock samples.
However, sequential HEPA vacuum sock samples indicated better collection efficiency on nonporous surfaces than wipe samples.
We used the average weight of five unused sock samples to estimate the presampling weight of the vacuum socks; the average weight of the unused socks was 0.70 g (standard deviation 0.02 g).
Disposable inserts may prevent cross-contamination of the vacuum nozzle or subsequent sock samples.
The HEPA vacuum sock samples were analyzed by an LRN contract laboratory.
Our study focused on sampling nonporous surfaces; under these circumstances, HEPA vacuum sock samples and wipe samples performed similarly.
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CEO of Professional Science Editing for Scientists @ prosciediting.com