Subjects were excluded if they did not have a routine serum sample collected within 4 h of presentation or were not admitted to the general ward after the DEM presentation.
In addition to clinical routine measurements, serum samples from patients were drawn directly before induction of anaesthesia (pre-OP), immediately before termination of surgery (intra-OP) and 24 h postoperatively (post-OP).
In addition to clinical routine measurements, serum samples were obtained after induction of anesthesia (preoperative), 45 min after institution of CPB, 2 min after opening of cross-clamp (myocardial reperfusion), 15 min after weaning from CPB, at 1 h after admission to the ICU, 6 h after admission, and 24 h after admission to the ICU for the laboratory determination of MIF, MIF-2, and sCD74.
Urine collection intervals thus included the time of routine serum creatinine sampling for ICUs in our institution.
Lack of clinical signs is a risk factor for PRRSV transmission, as specific surveillance measurements are usually not used, i.e. routine analysis of serum samples by PCR during the peak of viremia or after seropositive results allowing detection of the shedding of PRRSV in semen.
Serum samples for routine laboratory studies were obtained from the patients at the initial visit.
At HCB, as part of the routine workup for febrile patients, serum samples were collected, stored at −20°C, and transported in liquid nitrogen to Quito for PCR.
Nevertheless, after routine analysis, residual CSF and serum samples, stored throughout at 4°C, were made anonymous according to the general advice of the ethics committee of the Medical Faculty, University Goettingen.
To correlate CA-125 concentrations in serum samples collected for routine hCG measurements 11 ± 2 days after embryo transfer in relation to pregnancy and its outcome.
The appeal of using plasma miRNAs in clinical applications is high, as separation and preservation of plasma or serum samples is clinical routine also in M. tuberculosis endemic countries.
