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All suspension cell lines were maintained at concentrations between 2 × 105 and 12 × 105 cells/mL during routine culture and experiments, and were routinely tested for potential mycoplasma contamination.
Routine culture of E. coli strains was conducted in 2× yeast extract-tryptone (2xYT) medium.
GBS positivity rates by direct PCR and after routine culture were 15.7% (n = 192) and 12.6% (n = 154), respectively.
"I'm pretty sure that if I swabbed one of the vents and took it to the lab, and ran a routine culture on it, I'd find that some type of toxic mold was growing in the house".
Routine culture was performed by trypsinization of the cells twice a week.
For the purpose of routine culture, both cell lines were incubated at 37 °C under 5%% CO2 and 95%% humidity.
Brain heart infusion broth (BHI; Mast Group, Bootle, UK) with 2% sucrose was used for routine culture.
During routine culture, we observed that the proliferation rate of Clock knockout mESCs was much slower than that of the wild type mESCs.
We found that hPSCs highly express many SAC proteins that are also enriched in oocytes and preimplantation embryos, and can undergo abnormal mitosis during routine culture (Fig. 2A).
We are not, in fact, doing ourselves any favors by engaging in our routine culture of complaining about the government - not in this instance at least.
Lysogeny broth [45], [46] was used for the routine culture of all bacteria.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com