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After routine analysis, samples were kept at −80°C.
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After routine analysis all samples were stored at -20°C and thawed once before chemokine and cytokine analyses.
In the same way as for GC MS analysis, the initial validation data are continually being supplemented by performance data generated as part of analytical quality control during routine analysis of samples.
The developed stability-indicating analytical method can be used for the routine analysis of production samples, where sample load is higher and high throughput is essential for faster delivery of results.
It was used in routine analysis of environmental samples (wastewater, sludge, sand and oyster).
However we feel, this method has promising applications in the routine analysis of food samples in industry.
The possibility to transfer methods from thermal to differential-flow modulated comprehensive two-dimensional gas chromatographic (GC × GC) platforms opens interesting perspectives for routine analysis of complex samples.
Further work would be required to develop a suitably rapid and validated pipeline for routine analysis of such samples.
These can be performed easily on personal computers, and allow rapid and routine analysis of multiple samples.
The 250 μl volume was selected for routine analysis of plasma samples and the 125 μl volume was used when repeats were required.
The IFCC reference method is technically demanding, time consuming, and very expensive and is not designed for routine analysis of patient samples.
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