By conducting two rounds of splitting, i.e., the first round 100 kb from the right end of YAC CIC9e2 with pKY11 to generate 490 and 100 kb YACs and a second round 50 kb from the right end of the new 490 kb YAC to generate 440 and 50 kb YACs, the middle 50 kb region of a plant chromosome fragment harboring Kmr was successfully released as a split YAC.
The DNA constructs were made, according to [ 42], by two rounds of "Split-Primer" PCR, with the first PCR performed with a target protein-specific primer (5'-CCACCCACCACCACCAatgnnnnnnnnnnnnnnnn-3'; lowercase indicates the 5'-coding region of the target gene) and the AODA2306 primer.
Very rarely, a second round of splitting occurs, leading to identical quadruplets.
Occurring in only 1 in 11 million cases, identical quadruplets are created when a second round of splitting occurs after a fertilized egg has already split into two embryos.
Old men nurse cups of coffee, police officers linger over pastries and families crowd around sumptuous wedges of tarte Tropézienne: rounds of puffy brioche split and filled with pastry cream.
Arabidopsis also experienced two rounds of WGD after splitting from papaya (Freeling 2009).
The first round of post-split matches will be on the weekend of Saturday, 25 April, with the final round of matches on the weekend of 23/24 May.
Previous rounds of Afghan peace talks split the Taliban rank and file and the latest effort in Qatar collapsed in June when the government of President Hamid Karzai objected to the display of a Taliban flag outside its office in Doha and a plaque on the building inscribed: "Islamic Emirate of Afghanistan", the country's official name under the Taliban regime.
After four rounds of voting, the Hollywood split that shocked HuffPost Celebrity readers the most is... Heidi Klum and Seal's 2012 separation.
An extended pause between rounds because of a split in one of Clay's gloves — some say made purposely by Clay's trainer, Angelo Dundee — helped Clay recover, and in Round 5 he fulfilled his prediction, quickly reducing Cooper's face to a bloody mask.
After two rounds of fixtures, those leagues would split into three sections of eight for two rounds of matches.
