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This procedure involved several rounds of selecting for colony growth on agar containing citrate as the only carbon source.
As in a card game, the play involves developing a winning hand through rounds of selecting and discarding tiles.
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To try and evaluate if a pro-active MET approach, started from October 2013, based on daily hospital rounds of select patient groups, to try and identify at-risk patients, is more effective, when compared with the traditional, physiological-criteria based alert system, in reducing the overall Code Blue numbers in a tertiary care hospital in India.
The league's 30 clubs will run through seven rounds of picks, selecting the top previously undrafted 18- to 20-year-olds from Canadian junior teams, American colleges and European leagues.
There was an increase in phage TUs in subsequent rounds of selection indicating enrichment of selected anti-PLAP phage.
After 10 rounds of selection and amplification, selected aptamers have been characterised using a number of techniques, including fluorescence quenching, ELISA and competition ELISA procedures and a FRET type assay.
After eight rounds of selections, the final selected ssDNA pool was PCR-amplified and cloned into Escherichia coli using the TOTO TA cloning kit (Invitrogen, Carlsbad, CA).
Suppose a trait is not selected (multiple rounds of selection may be required; see section 9 in File S1 for more information); then the QTL is negligible or of no effect.
John Paul, moreover, has begun a second round of appointments, selecting prelates to succeed bishops he himself once appointed.
Deletion mutants were therefore subjected to fly transmission after each round of transfection, selecting for parasites that could adapt to a progressively thinner protein coat.
We took the evolved populations from the third and fifth rounds of the evolutionary runs selecting for medium expression and performed another round of selection on them, selecting 5% of the population closest to the desired log-fluorescence μ*.
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