Sentence examples for rounds of modified from inspiring English sources

Filamentous phage-displaying scFvs were selected against nmAb-KT and were enriched through five rounds of modified subtractive panning.

During different selection rounds of modified subtractive panning, bound scFv-containing phages were eluted easily and quickly from the immobilized nmAb-KT by using an elution buffer supplemented with HM-1 at concentration 10 μg/ml.

To examine the integrity of the library after the final rounds of modified subtractive panning, 50 individual colonies in E. coli TG1 were randomly isolated and the presence of the scFv DNA insert in these clones was confirmed by using polymerase chain reaction (PCR) amplification; these amplified products were analyzed on 4% agarose gels.

At least five unique genes (K1 - K5) were identified by nucleotide sequencing after the fifth round of modified subtractive panning.

After the fifth round of modified subtractive panning, the diversity of selected scFv clones was analyzed by fingerprint analysis of the scFv genes of the positive clones.

The phage-display library consisted of 2.8 × 1010 clones (CFU/ml) with a final diversity of 3.4 × 105 separate clones after fifth round of modified subtractive panning.

After the final fifth round of modified subtractive panning, 80% clones (40/50) were positive with a strong binding affinity to nmAb-KT.

To ascertain the scFv repertoire and the quality of the immunized antibody library, DNA segments encoding the scFv genes from 40 randomly selected positive clones (80%; 40/50) were identified after the fifth round of modified subtractive panning.