Sentence examples for rounds of evolutionary from inspiring English sources

Subsequent EMS mutagenesis, genome shuffling and selection in D-xylose-enriched D-xylose-enriched D-xylose-enrichedd by mulignocelluloseof evolutionary engineering in complex medium withydrolysate gradually established efollowed D-xylose fermentation.

Therefore, the recombinant strain HDY.GUF5 was subjected to several consecutive methodologies for strain improvement: mutagenesis, genome shuffling and selection, followed by multiple rounds of evolutionary adaptation for D-xylose fermentation.

However, in an earlier study, the triple hexokinase deletion strain continued to grow on glucose after several rounds of evolutionary engineering, and re-sequencing of this evolved strain revealed a mutant GAL1 sequence that rescued glucose phosphorylation in the triple hexokinase deletion strain thereby re-enabling growth on glucose [ 35].

Considerable improvement in the rate of D-xylose consumption was observed with each round of evolutionary adaptation.

To infer the values of μ* and τ that apply to our evolutionary runs, we performed a number of experiments in which we: Took a population from one of the rounds of our evolutionary runs.

Took a population from one of the rounds of our evolutionary runs.

We took the evolved populations from the third and fifth rounds of the evolutionary runs selecting for medium expression and performed another round of selection on them, selecting 5% of the population closest to the desired log-fluorescence μ*.

As shown in Figure 1B of the main paper, in the evolutionary runs in which we are selecting for high expression, the selection window is changing at every round of the evolutionary run.

Left panel: Excess noise levels of promoters from the third (black) and fifth (brown) round of the evolutionary run.

Middle panel: Excess noise levels of promoters from the third round of the evolutionary run (blue), and from clones that resulted from another round of either stringent (red), normal (yellow), or weak (green) selection.

As in Appendix 2 figure 3, the blue dots correspond to clones from the third and fifth round of the evolutionary run, the red dots result from another round of stringent selection (top 1%), the yellow dots from another round of standard selection (top 5%), and the green dots from a round of weaker selection (top 25%).