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Four rounds of cluster-sampled household surveys were conducted, the first of which took place from May until September 2004.
All differentially expressed miRNAs clustered together after five rounds of clustering.
All differentially expressed miRNAs in two libraries clustered together after 6 rounds of clustering.
Second, nucleotide consensus sequences of all 8310 contigs were subjected to two additional rounds of clustering using the program Sequencher (Gene Codes, Ann Arbor, Michigan).
Subsequent rounds of clustering followed by the extraction of representative sequences are conducted until the lowest selected identity value is reached or until all sequences are grouped in a single cluster.
At the beginning of every round of cluster head selection, all the sensors in a cluster provide the required information to their respective assistant node.
After which the current CH informs n CA to initiate the PSO computation for the next round of cluster head selection.
The number of rounds for cluster construction is equal to the clusters formed, which represents that only one CH is elected in each round.
Note that, because the lowest-energy conformer in each partition was used as an initial seed, low-energy conformers are more likely to be included than high-energy conformers when all partitions are combined together for next round of clustering.
This second round of clustering resulted in a Sanger-based database of 12,329 unigenes from Alexandrium.
The final clustering algorithm included three steps: (1) first round of clustering, (2) contact filtering, and (3) second round of clustering.
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