Sentence examples for round template from inspiring English sources

Each inner PCR reaction was performed with 32 replicates (40 μl each) using 5 μl of pooled first round template, and specific primers that incorporated a 4 base identification tag as well as a 19 base 454 adaptor sequence (Table S9).

The first-round template was subsequently used in nested second-round PCRs (Taq DNA polymerase, Roche, Burgess Hill, UK).

Additional details regarding the SELEX experiment, such as the number of selection rounds, template sequences and details on the selection solution may also be added.

If you don't have a compass, try tracing around a circle template, using something round such as a lid or a CD.

If you are planning a shape other than round, a template is essential: print it, cut out, place on the cake and cut round it with a sharp knife.

Using the products of the initial round as template, the second round of PCR was performed using primers "a" and "d" (or "e").

CapI-pb (108 bp) was recovered by semi-nested PCR with the primers ARTAYT (5'-GCNMGNACNGCNTAYAC-3') and WFQNRR, followed by ARTAYT and IAASLD (5'-TCSARNGARGCRGCDATC-3') with 1∶100 diluted product of the first round as template for a second PCR reaction.

In the third round, the template was 5 μL of mixture from the second round PCR, using the primer pair RPMS1-5/6, in a 50-μL reaction system.

Workflow Analysis was done after the first round of template bead preparation for each library according to the manufacturer's instructions using the Workflow Analysis kit from Applied Biosystems Life Technologies , Inc). to check library quality and the amount of templated beads generated per ePCR.

For internal standardization, a parallel real-time PCR targeted an invariant region in the converse exon within the same first-round amplicon template.

Additional file 3: Appendix C. Electronic health Record Interdisciplinary Rounds Note Template.