Sentence examples for round reactions from inspiring English sources

All 96 first round reactions for each time point were pooled and used as template for three inner PCR reactions.

Second round reaction mixes were identical to the first round and the sample was a 1 µl transfer from the first round reactions.

The PCR conditions for both the first and second round reactions were initial denaturation at 95°C for 10 min, followed by 35 cycles at 94°C for 30 s, 45°C for 35 s, and 72°C for 60 s.

First round reactions amplified a 4 kb product encoding Core, E1 and E2 structural proteins and a 7 kb product encoding the non-structural (NS2 5) proteins.

For each sample, the four first round reactions were pooled and 2 μl of this pool were used as template for second round PCR.

PCR first round reactions used Thermostart ABGene MasterMix (Fisher, Loughborough, UK), 0.25 μM forward primer, 0.25 μM reverse primer and 10 ng of converted DNA.

The PCR reaction contents of the second round reaction were the same as the first round, but the N. fuckeliana PCR primers were used and 1 50 dilution of the first-round PCR products were used as the DNA template for the second round PCR reaction.

Second round reaction conditions were as for the first round over 30 cycles.

Differential amplification occurred in the second round using the equivalent of 0.2 µL of the first round reaction as input.

To increase the yield and specificity of RACE-PCR products, a second nested round of PCR amplification was performed using 1 µl of the first round reaction.

Primers for HMPV F gene amplification were as described previously [13]. 2 µl cDNA was used in the first round and 1 µl first round product was used in the second round reaction.