Sentence examples for round products from inspiring English sources

First round products were amplified using 300 nM each primer (Alu-F 5'-GCCTCCCAAAGTGCTGGGATTACAG-3'; gag-R 5'-GTTCCTGCTATGTCACTTCC-3') and approximately 50 ng total DNA in a 25 µl reaction.

When the second round RT-PCR was performed after removing the wild-type sequences from the first round products by TaqI RE digestion (i.e., enrichment RT-PCR; RT-ePCR), all tested PBMCs showed the R46X mutation by direct sequencing (see Methods).

Second round products were generated with universal primers and products of the first round of amplification.

The third round of random PCR (25 μl) contained 1 μl of the second round products as template.

The second round products of the nested PCR for HBx were purified and subjected to gene sequencing analysis.

The primers used are shown together with the size of their respective 1st and 2nd round products (Table 1).

For example, for a round product, at least three cameras are used while use of five cameras has been reported in the literature [5].

For the second round PCR, a 1/100 dilution of the first round product was used as template.

Integrated HIV-1 DNA copies were determined in a nested qPCR using 5 µl of first round product as template.

Primers for HMPV F gene amplification were as described previously [13]. 2 µl cDNA was used in the first round and 1 µl first round product was used in the second round reaction.

For the second round of PCR, 1 μl from the first round product diluted 1/25 was used in each PCR reaction.