For the second round of selection, the cells selected at the first round were incubated, and β-glycosidases in cells were expressed.
After each round of selection, PCR amplification is employed to recover the selected mRNA to be used in the subsequent round.
Consequently, a single round of selection led to a single population of EGFP-positive cells.
The third or last round of selection is made after genotyping of BC3F2 or BC4F2 individuals.
The percentage transformation rate was calculated based on the explants surviving after the third round of selection.
Pacini: Some specific courses were available for the first round of selection, but I declined the offer.
As a result, a ∼12,000-fold enrichment of single-chain antibody (scFv) cDNA was achieved in a single round of selection.
Aptamer pools from each round of selection were tested for their binding to the target and non-target cells using flow cytometry.
Both mRNA and ribosome display have been successfully used to identify low-pmol/L affinity binders, even after only a single round of selection (Schilling et al., 2014).
The DNA pool collected after each round of selection was amplified by PCR, and the product was used to prepare ssDNA for the next round of selection.
The RT-PCR product was used for the next round of selection as described above.
