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This process represented one round of panning.
The generated phage particles were used in the next round of panning.
(A) The enrichment for phage particles in wells coated with antigen versus wells without antigen was detected after each round of panning.
This procedure constituted one round of panning.
Given this fact, we decided to work with phages from the first round of panning.
This trend, though not as pronounced, continued after the fourth round of panning.
The obtained products were applied to the affinity maturation or next round of panning.
The input phage number (1×1011 pfu) was kept constant for each round of panning.
After several washing steps, bound bacteria were detached and amplified before being subjected to a new round of panning.
The colonies were either used in a phage ELISA (see below) to identify antigen binders or they were pooled and used in another round of panning.
Similar(1)
A single-round of panning with high stringency was more successful and several full-sized scFvs were isolated, whose human-like character was quantified.
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