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The clarified medium was reused without any further nutrient supplementation, whereas a second round of medium reuse was performed after supplementation of main nutrients (phosphate sulfate nitrate), micronutrients and ferric chloride.
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The eyes are round and of medium size.
Following the final round of transduction, either fresh medium, medium containing 100 ng/ml doxorubicin or medium containing 10 μM nutlin-3 was used and the cells incubated for an additional 24 hours.
For each of the 4 lineages (L1 L4), the total number of generations over which Sup35 NM prion propagation is monitored corresponds to the number of cell divisions that occur in the absence of New1 during 4 rounds (R1 R4) of growth on solid medium and an additional round of growth in liquid medium.
The liver contained solid neoplastic areas composed of medium size round to larger epithelioid oval cells with abundant cytoplasm, vesicular nuclei, and single prominent nucleoli supported by a fibrovascular stroma.
Skin biopsy specimens and the swab were cultured in human embryonic lung fibroblasts by using the centrifugation shell-vial technique (3.7 mL; Sterilin Ltd., Felthan, UK); 12-mm round coverslips seeded with 1 mL of medium containing 50,000 cells and incubated in a 5% CO2 incubator at 37°C for 3 days were used to obtain a confluent monolayer (8 ).
Subsequently, the gene-transduced MD.45 population (MD.45-HER/ ζ) was selected for 4 days in culture medium containing 1 mg ml−1 G418, followed by an additional round of selection (5 days) in medium containing 0.4 mg ml−1 G418.
Moran is of medium height, with a round face and full cheeks, and he has a sandpapery beard and short hair like flecks of metal.
We randomly selected 479 promoters from the evolved set (72 medium expressers and 72 high expressers after three rounds of selection; 168 medium expressers and 167 high expressers after five rounds of selection) and quantified mean and variance in fluorescence.
A second round of selection on the same medium was performed, and the valid AD plasmids were removed.
Multiple bid/no-bid decisions were collected in two rounds via a designed bidding experiment involving managers of medium to large contractors based in Hong Kong and Singapore.
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