Sentence examples for round of integration from inspiring English sources

In the case of using error-prone PCR, subsequent iterations of selection would involve PCR amplification of the gene from the enriched cell population using error-prone conditions, followed by a new round of integration and selection.

Repeated rounds of integration allowed the transgene copy number to be increased.

After three rounds of integration, a high-producer CHO cell clone with six copies of the scFv-Fc gene was successfully established.

The counter selection system of the URA3 gene and 5-fluoroorotic acid (5-FOA) allows multiple rounds of integration of functional genes into the Y. lipolytica genome thereby to introduce many copies of foreign genes (Barth and Gaillardin 1996; Zhu et al. 2010).

The use of mutant lox sites for controlling reversibility would allow only two rounds of integrations as the recombination between a set of mutant lox sites generates a loxP [ 9], which can be targeted, but two cis-positioned lox sites will be generated in the resulting SSI that would complicate subsequent use of Cre.

The new round of political integration is being driven by the need to govern the euro zone better; but ten of the 27 are not part of it.

For the second round of gene integrations, we chose pOSB2xx as the donor vector.

A round of consolidation followed.

After two total rounds of transposon integration and recombination between the two DRs, only the exogenous DNA inserted into the MCS (passenger genes) and a single 430-bp scar sacBDR fragment remained in the chromosome after excision.

The resulting 'stacked' site, as expected, contained attL and attR between each construct, the number of which would increase with each round of site-specific integration.

The single recombination site in the SMG-free SSI site serves as the target for the next round of site-specific integration.