Sentence examples for round of expression from inspiring English sources
i. #More round of expression may be required depending on expression level.
A second Dox administration 48 h after the initial induction resulted in a second round of expression of green MitoTimer.
Over 40% of cloned genes were overexpressed in Escherichia coli BL21 (DE3 -RP codon plus strain in the first round of expression screening.
In sum, the second round of expression screening resulted in the production of all the target proteins which were not expressed in the first round of screening.
The stably transduced cells were then subjected to two rounds of AblPP induction and cells that remained attached after the second round of AblPP expression were collected (Fig. 1B).
In some experiments, Dox was added a second time (48 h later) to initiate a second round of MitoTimer expression, and cells were analyzed 12 32 h after the second Dox pulse.
The protein engineering stages were guided by B and T cell epitope prediction programs and available literature on ABD, and included iterative rounds of protein expression and analytical characterisation [ 104].
If this negative feedback loop were the entire story, one would anticipate only a single robust round of per/cry gene expression would occur, and then a steady, basal level of gene expression would persist.
After several rounds of genetic engineering of expression plasmids combined with metabolic engineering of P. putida, DHA production yields were eventually increased more than threefold.
In addition, the effects of an additional round of amplification on gene expression profiles have been reported to be minor on the basis of high Pearson correlation coefficients of all genes (0.85 to 0.94) between one and two rounds of amplification [ 4, 10, 11] and of consistent numbers of differentially expressed genes detected between one and two rounds of amplification [ 12].
After two sorting rounds of recombinant virus and measurements of expression efficiency for more than 20 viral clones, the over-expressed recombinant virus Bm-P12A3C was obtained.
