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To address the issue, we combined the proteins showing high sequence similarities by another round of BLAST searches, with a more stringent E-value threshold of 1E-120.
The single-copy status of primers was ensured by another round of BLAST queries to the A. thaliana genome.
The third round of BLAST (aligning the seeds to the expected members of the ad hoc families) took less than 300 CPU-hours.
The second round of BLAST searches were performed against the SwissProt, RefSeq and nr databases via the platform Blast2GO (Blast2GO
Genes that yielded a one:many relationship during the orthology search were subjected to an additional round of BLAST analysis.
We then used various validated sequences as queries for a second round of BLAST search on complete genomes.
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As he ran, he saw two workers still standing outside just as another round of blasts engulfed the construction site in flames.
Assembled sequences were BLAST searched against mitochondrial sequences, and BLAST-hit sequences were used as seeds for the next round of BLAST-assembly workflow.
phredPhrap (www.phrap.org) was used to assemble traces into contigs, and further rounds of blast searching and phredPhrap were used to extend contigs.
Two rounds of BLAST [ 18] are performed on each pair of genome sequences.
Hits with E-values less than or equal to 0.01 were used as query sequences in successive rounds of BLAST.
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